Internship Project
Biology and Neuroscience

Epigenetic and epitranscriptomic mechanisms of gene regulation in a unicellular eukaryote

Institution
Humboldt-Universität zu Berlin, Department of Biology
Subject Area
Molecular Biology, Biochemistry, Molecular Genetics
Availibility
Position filled
Project Supervisor(s)
Prof. Dr. Ann Ehrenhofer-Murray
Academic Level
Advanced undergraduate students (at least 2nd year)
Graduate students

Language
English
Further Information
Project Type
Academic Research

Project Content
Our laboratory studies the regulation of gene expression and protein translation by modifications in chromatin and RNA using two unicellular eukaryotes, the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, as model organisms. We are particularly interested in understanding how modifications of the centromeric histone variant CENP-A regulate the recruitment of kinetochore proteins to the centromere, because defects in this process cause chromosome missegregation, a hallmark of cancer cells. Furthermore, we are studying the modification of RNAs with queuine, a guanine analog that is obtained by eukaryotes from bacterial sources. We are investigating the RNAs carrying this modification and how it affects RNA function. Defects in RNA modifications are the cause of human genetic disorders. 
We offer projects in these areas that employ methods of molecular biology, molecular genetics and biochemistry in order to obtain mechanistic insights into how protein and RNA modifications regulate basic cellular function.
Tasks for Interns
  • Experimental work in molecular biology, biochemistry and genetics using yeast and bacteria. 
  • Cloning, genetic crosses, screening, sequencing.
Academic Level
Advanced undergraduate students (at least 2nd year)
Graduate students

Requirements
  • Knowledge in chromatin and epigenetics; 
  • Basic experience in molecular biology (restriction digestion, DNA isolation, cloning) at advanced bachelor or Master level;
  • Interest in basic scientific questions and work with a microorganism.
Expected Preparation
Review of relevant scientific literature.
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For more information on the Humboldt Internship Program or the project, please contact the program coordinator.